氫氣治療急性胰腺炎

 

詳細資料請下載全文:全文下載

本文來自長征醫院普通外科王強主任課題組,被BBRC接受。

急性胰腺炎目前最有效的治療方法是用胰酶抑製劑,但費用非常昂貴,尋找更好的廉價治療方法仍是臨床關注的研究熱點。本研究用含氫生理鹽水治療動物急性胰腺炎,用形態學方法證明能減輕胰腺組織損傷,減少NFKb的水平,減少細胞凋亡數量,並能促進胰腺腺細胞的增殖,採用澱粉酶和氧化損傷指標分別證明對胰腺功能和氧化損傷均有明顯的改善作用,雖然研究的指標比較單薄。但本文首次在國際上證明氫氣能有效治療急性胰腺炎,為這一重要疾病的治療提供了新有效手段的前景。希望將來在臨床上能實現,將造福患者,降低費用。

Hydrogen-rich saline ameliorates the severity of L-arginine-induced acute pancreatitis in rats

ABSTRACT

Background and Aims: Acute pancreatitis is an inflammatory process involving pancreatic tissue edema, acinar cell damage, 

Methods: Acute pancreatitis was induced in Sprague-Dawley rats by giving two intraperitoneal injections of Arg, each at concentrations of 250 mg/ 100 gbody weight, with a 1 h interval between injections. Rats were divided randomly into three groups Normal, Arg plus saline treatment, and Arg plus hydrogen-rich saline treatment groups. Hydrogen-rich saline ( > 0.6 mM, 6 ml/kg) or saline (6 ml/kg) was administered respectively via tail vein in 15 minutes after each Arg administration. Pancreatitis severity was assessed by analysis of serum amylase, pancreatic water content, and histology. Samples of pancreas were also taken for measuring malondialdehyde and myeloperoxidase. Apoptosis in pancreatic acinar cell was determined with terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling technique (TUNEL). Expression and distribution of proliferating cell nuclear antigen (PCNA) and nuclear factor kappa B ( NF-κB) were detected with immunohistochemistry.

Results: Hydrogen-rich saline treatment significantly attenuated the severity of Arg-induced acute pancreatitis with inhibiting Arg-induced apoptosis, pancreatic tissue edema, activation of NF-κB and promoting acinar cellsproliferation. Moreover, Hydrogen-rich saline treatment significantly limited the neutrophil infiltration , lipid oxidation and ameliorated the increased serum amylase.

Conclusions: These results indicate that hydrogen treatment has a protective effect against acute pancreatitis. This protective effect is possibly due to its ability to inhibit oxidative stress, apoptosis and to promote acinar cell proliferation. 

Keywords: Hydrogen, Acute pancreatitis, Antioxidant, Oxidative stress, Apoptosis

 目前已經發表的氫氣生物學效應的論著統計,紅色為中國論文,蘭色為日本,黑色為美國

    1.          Ohsawa I, Ishikawa M, Takahashi K, et al. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med, 2007; 13: 688-94. pdf

2.          Fukuda KI, Asoh S, Ishikawa M, et al. Inhalation of hydrogen gas suppresses hepatic injury caused by ischemia/reperfusion through reducing oxidative stress. Biochem Biophys Res Commun, 2007; 361: 670-4 pdf

3.          Buchholz BM, Kaczorowski DJ, Sugimoto R, et al. Hydrogen inhalation ameliorates oxidative stress in transplantation induced intestinal graft injury.Am J Transplant. 2008; 8(10):2015-24 pdf

4.          Cai JM, Kang ZM, Liu W, et al. Hydrogen therapy reduces apoptosis in neonatal hypoxia-ischemia rat model. Neurosci lett 2008,441(2):167-72 pdf

5.          Hayashida K, Sano M, Ohsawa I, et al. Inhalation of hydrogen gas reduces infarct size in the rat model of myocardial ischemia-reperfusion injury. Biochem Biophys Res Commun, 2008,373(1):30-5. pdf

6.          Kajiyama S, Hasegawa G, Asano M, et al. Supplementation of hydrogen-rich water improves lipid and glucose metabolism in patients with type 2 diabetes or impaired glucose tolerance, Nutr. Res, 2008,28:137-43 pdf

7.          Ohsawa I, Nishimaki K, Yamagata K, et al. Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice.Biochem Biophys Res Commun, 2008, 377(4):1195-8 pdf

8.          Sato Y, Kajiyama S, Amano A, et al. Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice. Biochem Biophys Res Commun, 2008; 375(3):346-50. pdf

9.          Nagata K, Nakashima-Kamimura N, Mikami T, et al. Consumption of Molecular Hydrogen Prevents the Stress-Induced Impairments in Hippocampus-Dependent Learning Tasks during Chronic Physical Restraint in Mice. Neuropsychopharmacology. 2009; 34(2):501-8. pdf

10.       Cai JM, Kang ZM, Liu K, et al. Neuroprotective effects of hydrogen saline in neonatal hypoxia-ischemia rat model. Brain Res. 2009;1256:129-37. pdf

11.       Nakashima-Kamimura N, Mori T, Ohsawa I, Asoh S, Ohta S.Molecular hydrogen alleviates nephrotoxicity induced by an anti-cancer drug cisplatin without compromising anti-tumor activity in mice.Cancer Chemother Pharmacol. 2009; 64(4):753 -61. pdf

12.       Zheng XF, Mao YF, Cai JM ,Li YH, Liu WW, Sun PL, Zhang JH, Sun XJ, Yuan.HB Hydrogen-Rich Saline Protects against Intestinal Ischemia/Reperfusion Injury in Rats Free Radical Res.2009; 43(5): 478-84. pdf

13.       Fu Y, Ito M, Fujita Y, et al. Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson’s disease. Neurosci lett. 2009; 453(2):81-5.pdf

14.       Matchett GA, Fathali N, Hasegawa Y, et al. Hydrogen gas is ineffective in moderate and severe neonatal hypoxia-ischemia rat models.Brain Res. 2009; 1259:90-7 . pdf

15.       Mao YF, Zheng XF, Cai JM ,et al. Hydrogen-rich saline reduces lung injury induced by intestinal ischemia/reperfusion in rats. Biochem Biophys Res Commun.2009; 381(4):602-5. pdf

16.       Kikkawa YS, Nakagawa T, Horie RT, Ito J.Hydrogen protects auditory hair cells from free radicals.Neuroreport. 2009;20(7):689-94. pdf 

17.       Kajiya M, Sato K, Silva MJ, Ouhara K, Do PM, Shanmugam KT, Kawai T. Hydrogen from intestinal bacteria is protective for Concanavalin A-induced hepatitis. Biochem Biophys Res Commun. 2009; 386(2):316-21. pdf

18.       Kajiya M, Silva MJ, Sato K, Ouhara K, Kawai T. Hydrogen mediates suppression of colon inflammation induced by dextran sodium sulfate. Biochem Biophys Res Commun. 2009 Aug 14;386(1):11-5. 2009; 386(1) :11-5. pdf

19.       Sun Q, Kang ZM, Cai JM, Liu WW, Liu Y. Zhang JH, Denoblec PJ, Tao HY, Sun XJ.Hydrogen-rich saline protects myocardium against ischemia/reperfusion injury in rats Exp Biol Med.2009; in press pdf 

20.       Chen H, Sun YP, Hu PF, Liu WW, Xiang HG, Li Y,Yan RL, Su N, Ruan CP, Sun XJ, Wang Q.The effects of hydrogen-rich saline on the contractile and structural changes of intestine induced by ischemia-reperfusion in rats. J Surg Res.2009; in press pdf

21.      Oharazawa H et al.Rapid Diffusion of Hydrogen Protects the Retina: Administration to the Eye of Hydrogen-Containing Saline in Retinal Ischemia-Reperfusion Injury.   Investigative Ophthalmology & Visual Science 2010;51:487-492  pdf

22.       Itoh T, et al. Molecular hydrogen suppresses FcεRI-mediated signal transduction and preventsdegranulation of mast cells. Biochem Biophys Res Commun. 2009; 389: 651-656  pdf

23.       Fujita K, Seike T, Yutsudo N, Ohno M, Yamada H, et al. Hydrogen in Drinking Water Reduces Dopaminergic Neuronal Loss in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Mouse Model of Parkinson’s Disease . PLoS ONE. 2009; 4(9): e7247. doi:10.1371/journal.pone.0007247 pdf

24.       Xie KL et al. Protective Effects of Hydrogen Gas on Murine Polymicrobial Sepsis via Reducing Oxidative Stress and HMGB1 Release. Shock 2009 pdf

25.       Qian LR et al. Radioprotective effect of hydrogen in cultured cells and mice. Free Radical Res. 2009 pdf

26.     Cardinal JS, Zhan J, Wang Y, Sugimoto R, Tsung A, McCurry KR, Billiar TR, Nakao A. Oral hydrogen water prevents chronic allograft nephropathy in rats. Kidney Int. 2010;77(2):101-9. pdf 

27.      Y. Saitoh, Y. Yoshimura, K. Nakano, N. Miwa. Platinum nanocolloid-supplemented hydrogendissolved water inhibits growth of human tongue carcinoma cells preferentially over normal cells.Experimental Oncology 31, 156–162, 2009 pdf

 28.   Nakao, A, Kaczorowski, DJ, Wang, Y, Cardinal, JS, Buchholz, BM, Sugimoto, R, Tobita, K, Lee, S, Toyoda, Y, Billiar, TR, McCurry, K R. Amelioration of rat cardiac cold ischemia/reperfusion injury with inhaled hydrogen or carbon monoxide, or both. J Heart Lung Transplant. 2009; in press

 

neutrophil infiltration and the rapid production and release of inflammatory cytokines, which play a dominant role in local pancreatic inflammation and systemic complications. Molecular hydrogen, which reacts with the hydroxyl radical, a highly cytotoxic species produced in inflamed tissues, has been considered as a novel antioxidant. The study was to evaluate the therapeutic effects of hydrogen-rich saline on the L-arginine(Arg)-induced acute pancreatitis. pdf

 

 

 


 

  

 

台灣氫水實驗室 TEL:0963-210-763
email: hworker77086600@yahoo.com.tw
台灣氫水實驗室部落格:http://blog.xuite.net/hworker77086600/twblog

歡迎來電索取氫水辨認方法(防止喝到傷身的假氫水)

提供玻璃瓶裝飽和氫水供對比,免費兩瓶給你測試,收運費120元。(運費是宅配業者收去的)

 

 

 

 

Copyright © 2017 台灣低氘"氫水"實驗室|維護: wpweaver