LTP電生理指標，確定了治療效果，這個研究的指標和新意都比較突出，發表在 BR上比較委屈。將來這個文章將會有比較好的引用情況。因為這個領域將是比較重要的研究方向。一是這個疾病比較重要，另一個是抗炎症是這個疾病將來比較有前途的治療手段。如果氫氣在這個疾病能得到臨床證據，將是比較有前景的。

這個工作具有非常好的原始創新性。值得閱讀，值得關注，值得推薦。

日本醫科大學太田小組目前也在開展老年性癡呆氫氣水治療的研究，他們在剛舉行的學術會議中已經介紹了該工作。我們這個研究是採用腦內註射 amyloid β的癡呆模型，腹腔注射氫氣鹽水，採用炎症因子測定，行為學評價，氧化損傷指標和 

Hydrogen-rich Saline Improves Memory Function in a Rat Model of Amyloid-beta-induced Alzheimer’s Disease by Reduction of Oxidative Stress

Jian LI a , Cai WANG a , John H. Zhang b , Jian-mei CAI c , Yun-peng CAO a , Xue-jun SUN c

a Department of Neurology, the first affiliated hospital of China Medical University, Shengyang 110001, China; b Department of Neurosurgery, Loma Linda University, California, CA, USA; cDepartment of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, PR China

Corresponding authors: Yun-peng CAO, Department of neurology, First afflicted Hospital, ChinaMedical University, Shenyang, 110001, PR China.

Telephone: (86)2483282516, Fax: (86)2483282385. E-mail: ypengcao@yahoo.com;

Xue-jun Sun, Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, 200433, PR China. Tel: (86)2125070349. Fax: (86)2165492382. Email: sunxjk@hotmail.com

Abstract

This study is to examine if hydrogen-rich saline reduced amyloid β (Aβ) induced neural inflammation, and learning and memory deficits in a rat model. SD male  rats (n = 84, 280 -330g) were divided into three groups, sham operated, Aβ1 -42 injected and Aβ1-42 plus hydrogen-rich saline treated animals. Hydrogen-rich saline ( 5 ml/kg, ip, daily ) was injected for 14 days after intracerebroventricular injection of Aβ1-42. The levels of MDA, IL-6 and TNF-α were assessed by biochemical and ELISA analysis. Morris Water Maze and open field task were used to assess the memory dysfunction and motor dysfunction, respectively. LTP were used to detect the electrophysiology changes, HNE and GFAP immunohistochemistry were used to assess the oxidative stress and glial cell activation. After Aβ1-42 injection, the levels of MDA, IL-6, and TNF-α were increased in brain tissues and hydrogen-rich saline treatment suppressed MDA, IL-6, and TNF-α concentration. Hydrogen-rich saline treatment improved Morris Water Maze and enhanced LTP in hippocampus blocked by Aβ1-42. Furthermore, hydrogen-rich saline treatment also decreased the immunoreactivitiy of HNE and GFAP in hippocampus induced by Aβ1-42. In c onclusion ,hydrogen-rich saline prevented Aβ-induced neuroinflammation and oxidative stress, which may contribute to the improvement of memory dysfunction in this rat model.

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