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本文來自長征醫院普通外科王強主任課題組，被BBRC接受。
急性胰腺炎目前最有效的治療方法是用胰酶抑製劑，但費用非常昂貴，尋找更好的廉價治療方法仍是臨床關注的研究熱點。本研究用含氫生理鹽水治療動物急性胰腺炎，用形態學方法證明能減輕胰腺組織損傷，減少NFKb的水平，減少細胞凋亡數量，並能促進胰腺腺細胞的增殖，採用澱粉酶和氧化損傷指標分別證明對胰腺功能和氧化損傷均有明顯的改善作用，雖然研究的指標比較單薄。但本文首次在國際上證明氫氣能有效治療急性胰腺炎，為這一重要疾病的治療提供了新有效手段的前景。希望將來在臨床上能實現，將造福患者，降低費用。
Hydrogen-rich saline ameliorates the severity of L-arginine-induced acute pancreatitis in rats
ABSTRACT
Background and Aims: Acute pancreatitis is an inflammatory process involving pancreatic tissue edema, acinar cell damage,
Methods: Acute pancreatitis was induced in Sprague-Dawley rats by giving two intraperitoneal injections of Arg, each at concentrations of 250 mg/ 100 gbody weight, with a 1 h interval between injections. Rats were divided randomly into three groups : Normal, Arg plus saline treatment, and Arg plus hydrogen-rich saline treatment groups. Hydrogen-rich saline ( > 0.6 mM, 6 ml/kg) or saline (6 ml/kg) was administered respectively via tail vein in 15 minutes after each Arg administration. Pancreatitis severity was assessed by analysis of serum amylase, pancreatic water content, and histology. Samples of pancreas were also taken for measuring malondialdehyde and myeloperoxidase. Apoptosis in pancreatic acinar cell was determined with terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling technique (TUNEL). Expression and distribution of proliferating cell nuclear antigen (PCNA) and nuclear factor kappa B ( NF-κB) were detected with immunohistochemistry.
Results: Hydrogen-rich saline treatment significantly attenuated the severity of Arg-induced acute pancreatitis with inhibiting Arg-induced apoptosis, pancreatic tissue edema, activation of NF-κB and promoting acinar cellsproliferation. Moreover, Hydrogen-rich saline treatment significantly limited the neutrophil infiltration , lipid oxidation and ameliorated the increased serum amylase.
Conclusions: These results indicate that hydrogen treatment has a protective effect against acute pancreatitis. This protective effect is possibly due to its ability to inhibit oxidative stress, apoptosis and to promote acinar cell proliferation.
Keywords: Hydrogen, Acute pancreatitis, Antioxidant, Oxidative stress, Apoptosis
目前已經發表的氫氣生物學效應的論著統計，紅色為中國論文，蘭色為日本，黑色為美國
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neutrophil infiltration and the rapid production and release of inflammatory cytokines, which play a dominant role in local pancreatic inflammation and systemic complications. Molecular hydrogen, which reacts with the hydroxyl radical, a highly cytotoxic species produced in inflamed tissues, has been considered as a novel antioxidant. The study was to evaluate the therapeutic effects of hydrogen-rich saline on the L-arginine(Arg)-induced acute pancreatitis. pdf
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