氫氣和納米鉑的協同效應
已有1158次閱讀 2012-8-11 11:26 | 個人分類: 氫氣細胞學研究 | 系統分類: 科研筆記 | 關鍵詞:納米氫氣office style
納米鉑是納米尺度的金屬鉑顆粒，由於具有非常良好的比表面積，結合鉑的催化活性，研究發現該物質具有良好的抗氧化活性。氫氣和納米鉑聯合具有更理想的抗氧化作用，過去曾經發現兩者聯合使用具有抗腫瘤作用，本研究主要從紅細胞氧化損傷和細胞膜完整性以及血液流動性等角度觀察氫氣和納米鉑的協同效應。
聚乙烯吡咯烷酮（可作為血漿代用品）包埋的納米鉑可以分散到水中，也可以分散到富氫水中(氫氣濃度0.82 ppm;氧化還原電位-583mv，沒有飽和？或者氫氣的溶解度本來就這樣低)。本研究評價納米鉑分散富氫水和納米鉑水對1，1-二苯基-2-三硝基苯肼(DPPH)自由基的清除能力，以及對2,2′-偶氮(2-脒基丙烷)二氯化氫(AAPH)誘導的氧化應激後血液流動性的影響。使用電子自旋共振技術研究發現，當納米鉑濃度達到0.25-0.5 ppm時，正常水和氫氣水使DPPH自由基可分別下降77.5-59.6%或16.1-5.6%，如果沒有納米鉑，氫氣水只可以使DPPH自由基下降到81.3%（這個結果說明納米鉑具有清除自由基的作用，，儘管氫氣本身也有一定作用，但氫氣和納米鉑聯合可以大大促進這種作用，也就是說具有協同作用） 。
採用AAPH誘導氧化應激，將馬血和正常水或氫氣水共同孵育24小時後，馬血通過血液流變儀微隧道的時間分別為13.7 sec (100%)或5.7 sec (42.3%)。當聯合用0.5-1.0 ppm納米鉑時，馬血通過血液流變儀微隧道的時間分別為9.7-7.3 sec (71.6-53.8%)或4.3-1.3 sec (32.8-10.3%)。（和抗自由基的作用方式類似）。紅細胞聚集的比例從無納米鉑時的42.8%下降到23.8-21.0% or 15.8-9.8%,。使用電子掃描顯微鏡研究發現，AAPH誘導氧化應激可使紅細胞膜失去完整性，伴隨細胞膜無中空突起，顯示紅細胞的膜流動性受損。紅細胞聚集的比例達到46.2%。氫氣水可以使紅細胞聚集的比例降低到29.6%。而1.0 ppm納米鉑可以使使紅細胞聚集的比例降低到24.1%，氫氣水和1.0 ppm納米鉑聯合作用為21.1%。
研究結果現實，氫氣和納米鉑具有協同效應，可以聯合使用於氧化應激類疾病的治療。
J Nanosci Nanotechnol 2012 May;12 (5): 4019-27. [IF:1.352]
Colloidal platinum in hydrogen-rich water exhibits radical-scavenging activity and improves blood fluidity.
Kato S , Hokama R , Okayasu H , Saitoh Y , Iwai K , Miwa N .
Faculty of Life and Environmental Sciences, Prefectural University of Hiroshima, Nanatsuka 562, Shobara, Hiroshima 727-0023, Japan.
Abstract
The ‘colloidal platinum’ stabilized with polyvinylpyrrolidone (Pt/PVP-colloid) was dispersed in hydrogen-rich water (HW; hydrogen concentration, 0.82 ppm; oxidation-reduction potential, -583 mV) or regular water (RW; <0.01 ppm, +218 mV). And we evaluated the antioxidant activity of Pt/PVP-colloid in HW or RW on 1,1-diphenyl-2-picrylhydrazyl radical scavenging and improvement of blood fluidity under 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress.
When applied with the 0.25-0.5 ppm Pt/PVP-colloid in RW or HW, the level of DPPH radicals decreased to 77.5-59.6% or 16.1-5.6%, in contrast to the level as high as 81.3% for HW alone, respectively , as measured by an electron spin resonance method.
The horse blood, which was subjected to AAPH-induced oxidative stress, was incubated for 24 hr with RW or HW, and thereafter required 13.7 sec (100%) or 5.7 sec (42.3%) for passing through the micro-channels in a rheology equipment. When treated with 0.5-1.0 ppm Pt/PVP-colloid in RW or HW, the blood passage time in the micro-channels decreased dose-dependently to 9.7-7.3 sec (71.6-53.8%) or 4.3-1.3 sec (32.8 -10.3%), and the rate of micro-channels clogged with erythrocyte aggregates decreased to 23.8-21.0% or 15.8-9.8%, respectively, from 42.8% for no addition of Pt/PVP. By scanning electron microscopy, AAPH-treated erythrocytes lost intact surface morphology on the membrane together with protrusions and without hollows, being indicative of impaired transforming ability, and the rate of erythrocyte agglutination was increased to 46.2%. When treated the horse blood with HW alone significantly decreased the rate of erythrocyte agglutination to 29.6 %, whereas 1.0 ppm Pt/PVP-colloid in RW or HW decreased it to 24.1% or 21.1%, respectively. Thus, DPPH-radical-scavenging and erythrocyte-protecting effects of Pt/PVP-colloid in HW were superior to those of Pt/PVP-colloid in RW or Pt/PVP-free HW. The results could be mainly attributed to the enhanced antioxidant activity of Pt/PVP in HW, which may be due to captured-hydrogen on platinum.
PMID: 22852342 [Pubmed – In-Process]
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