氫氣生理鹽水對急性一氧化碳中毒的治療作用

已有1211次閱讀 2012-8-15 11:19 | 個人分類: 氫氣生理鹽水 | 系統分類: 論文交流 | 關鍵詞:氫氣的醫學

氫氣抗氧化抗炎症的研究已經有很多，關於氫氣治療一氧化碳中毒的研究，我們過去曾經發表在急救醫學雜誌上，我們證明氫氣對一氧化碳中毒引起的腦組織脫髓鞘改變，其原因可能和氫氣的抗氧化抗炎症有關。2年前我曾經到第四軍醫大學航空臨床醫學教研室介紹過氫氣的研究進展，他們對這一研究也十分有興趣，於是從那時就開展了相關研究，經過2年多的研究，這個課題組發表了第一篇關於氫氣治療一氧化碳中毒的研究論文。這一研究比較有新意的內容是關於氫氣對鐵銅和鋅離子的影響，另外關於細胞壞死和自噬的形態學研究也屬於比較新的研究內容。該論文來自第四軍醫大學航空臨床醫學教研室的研究，發表在急救醫學雜誌上。

氫氣作為抗氧化物質可保護腦缺血後自由基引起的氧化損傷，一氧化碳中毒後神經細胞損傷部分原因是自由基增加引起的氧化損傷，本研究的假說為氫氣可以保護一氧化碳中毒引起的神經損傷。研究採用氫氣生理鹽水治療大鼠一氧化碳中毒。雄性SD大鼠製備成一氧化碳中毒模型，氫氣生理鹽水每天腹腔注射6 ml/kg。使用檢測學習記憶能力的水迷宮和檢測動物認知能力的曠場實驗。行為學研究結束後，動物被斷頭處死，採用火焰原子吸收光譜測定法對血清和腦組織中的銅、鋅和鐵含量進行檢測。採用常規的HE和免疫組織化學染色對神經細胞壞死、調亡和自噬進行分析。研究結果發現，氫氣生理鹽水治療可以治療一氧化碳中毒引起的動物認知障礙，減少腦組織神經細胞壞死、調亡和自噬。另外，氫氣生理鹽水治療可以減少血清和腦組織中和自由基代謝關係密切的金屬離子鐵的含量，提高血清中銅的含量。研究結果表明，氫氣生理鹽水可以有效治療急性一氧化碳中毒後腦損傷，其治療機制可能通過改變某些微量元素減少自由基引起氧化損傷有關係。

Effects of Hydrogen-rich Saline on Rats with Acute Carbon Monoxide Poisoning.pdf

Effects of Hydrogen-rich Saline on Rats with Acute Carbon Monoxide Poisoning

Wenlan Wang, PhD ∗ , Liang Tian, MM ∗ , Ya Li, MM†, Xiaojun Wang, MM†, Feng Xia, MM ∗ , Li Li, MM ∗ , Jinsheng Li, PhD†, Zuoming Zhang, PhD ∗ ,

∗ Department of Clinical Aerospace Medicine, School of Aerospace Medicine, Fourth Military Medical University, Xi’ an, Shaanxi Province, China

† Department of Aerospace Hygiene and Health Service, School of Aerospace Medicine, Fourth Military Medical University, Xi’ an, Shaanxi Province, China

Received 21 April 2011. Revised 3 August 2011. Accepted 19 January 2012. Available online 13 August 2012.

http://dx.doi.org/10.1016/j.jemermed.2012.01.065, How to Cite or Link Using DOICited by in Scopus (0)Permissions & Reprints

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Abstract

Background

Studies have shown that inhalation of hydrogen gas, which acts as an antioxidant, can protect the brain against free radicals in rats with ischemia-reperfusion. The neuronal damage caused by acute carbon monoxide (CO) poisoning is partly free radical mediated. We hypothesize that hydrogen may prevent neurological damage from CO poisoning.

Objectives

This study is designed to test whether hydrogen (H2)-rich saline will have a protective effect on rats with acute CO poisoning.

Methods

Male Sprague-Dawley rats were subjected to CO poisoning. H2-rich saline was administered by peritoneal injection (6 mL/kg/24 h). We used the Morris water maze and the open field test to determine cognitive function. After cognitive function studies , rats were decapitated and the levels of trace elements copper (Cu), zinc (Zn), and iron (Fe) in serum and brain were assessed by flame atomic absorption spectrometry. Necrosis, apoptosis, and autophagy of neurons were assessed by HE staining and immunohistochemical staining in another group of rats.

Results

H2-rich saline treatment improved the cognitive deficits and reduced the degree of necrosis, apoptosis, and cell autophagy in rats. Additionally, H2-rich saline decreased the content of Fe in serum and brain in these rats, and increased the content of serum Cu related to free radical metabolism.

Conclusions

H2-rich saline may effectively protect the brain from injury after acute CO poisoning. The mechanism of this protection may be related to lessening oxidative damage by affecting trace elements in vivo.

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