低氘氫水實驗室

日本學者07年 Nature Med （ Ohsawa et al. 2007 ）文章發表後，立刻引起我們的極大興趣。我們採用不同的腦缺血模型，驗證呼吸 2% 氫氣是否可治療腦缺血損傷，研究結果與日本學者的結果類似，證明呼吸 2% 氫氣確實能夠治療新生兒缺血缺氧後損傷。該內容已發表文章，見 Cai JM, Kang ZM, Liu W, et al. Hydrogen therapy reduces apoptosis in neonatal hypoxia-ischemia rat model. Neurosci lett 2008,441(2):167-172. 文章發表後，立刻引起關注，目前已經被引用 6次。這個文章是我們的第一篇關於氫的文章, 比日本學者第一篇晚了1年. 但是我們的速度也是比較快的

Abstract: Hypoxia-ischemia (HI) brain injury is a major cause of neuronal cell death especially apoptosis in the perinatal period. This study was designated to examine the effect of hydrogen therapy on apoptosis in an established neonatal HI rat pup model. Seven-day -old rat pups were subjected to left common carotid artery ligation and then 90 min hypoxia (8% oxygen at 37 °C). Immediately after HI insult, pups were placed into a chamber filled with 2% H2 for 30 min, 60 min, or 120 min, respectively. 24 hr after 2% H2 therapy, the pups were decapitated and brain injury was assessed by 2,3,5-triphenyltetrazoliumchloride (TTC), Nissl, and TUNEL staining, as well as caspase-3, caspase- 12 activities in the cortex and hippocampus. H2 treatment in a duration dependent manner significantly reduced the number of positive TUNEL cells and suppressed caspase-3 and -12 activities. These results indicated H2 administration after HI appeared to provide brain protection via inhibition of neuronal apoptosis . 

被引用文獻目錄

Ohsawa I, Nishimaki K, Yamagata K, et al. Consumption of hydrogen water prevents atherosclerosis in apolipoprotein E knockout mice.Biochem Biophys Res Commun, 2008, 377(4):1195-8

Ohta S.Hydrogen gas and hydrogen water act as a therapeutic and preventive antioxidant with a novel concept Nippon Ronen Igakkai Zasshi. 2008,45(4):355-62.

Taniguchi H, Andreasson K.The hypoxic-ischemic encephalopathy model of perinatal ischemia.J Vis Exp. 2008 Nov 19;(21) pii: 955. doi: 10.3791/955

Cai JM, Kang ZM, Liu K, et al. Neuroprotective effects of hydrogen saline in neonatal hypoxia-ischemia rat model. Brain Res. 2008 Nov 27. [Epub ahead of print]

Nakashima-Kamimura N, Mori T, Ohsawa I, Asoh S, Ohta S.Molecular hydrogen alleviates nephrotoxicity induced by an anti-cancer drug cisplatin without compromising anti-tumor activity in mice.Cancer Chemother Pharmacol. 2009 Jan 16. [Epub ahead of print]

Gerald A. Matchett, Nancy Fathali, Yu Hasegawa, et al. Hydrogen gas is ineffective in moderate and severe neonatal hypoxia-ischemia rat models, Brain Res. 2009, doi:10.1016/j.brainres.2008.12.066

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